Vol. 8, Issue 4 (2020)
Molecular characterization of cotton (Gossypium hirsutum L.) using simple sequence repeat (SSR) markers
Author(s): Anjani A, Padma V, Ramana JV and Satish Y
Abstract: Cotton is a major commercial crop with vast and diversified uses of its products. The improvement of any crop mainly depends upon the nature and magnitude of genetic variability present in the base population. The objective of this study was to assess molecular characterization among the 40 G. hirsutum genotypes employing SSR markers. A total of 50 SSR markers were used for analysis among which 19 showed polymorphism with a total of 47 SSR alleles which were produced across 19 loci with estimated fragment length of 100 to 700 bp. The number of alleles varied from 2 to 4 with average number of alleles per primer pair was 2.47. The PIC values ranged from 0.36 (BNL 3445) to 0.98 (CCRI 0230) with an average of 0.67 indicating more informativeness of the primers used. The general cluster analysis was performed using the unweighted pair-group method on arithmetic averages (UPGMA) method based on similarity coefficients and the 40 genotypes were grouped into 7 clusters among which three were solitary clusters. Jaccard’s similarity matrix was constructed using binary data. Similarity coefficients among 40 genotypes ranged from 0.03 (between RAH 1033 and L 788) to 0.80 (TCH 1741 and ARBH 1402) indicating vast genetic base. The genetically diverse genotypes having least similarity coefficient value, RAH 1033 and L 788, can be used in future hybridization programme to exploit heterosis.
DOI: 10.22271/chemi.2020.v8.i4ar.10197
Pages: 3518-3522 | 353 Views 87 Downloads
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How to cite this article:
Anjani A, Padma V, Ramana JV, Satish Y. Molecular characterization of cotton (Gossypium hirsutum L.) using simple sequence repeat (SSR) markers. Int J Chem Stud 2020;8(4):3518-3522. DOI: 10.22271/chemi.2020.v8.i4ar.10197
