Vol. 8, Issue 6 (2020)
Assessment of genetic relationship among varieties of finger millet using ISSR and SSR markers
Author(s): S Yadav, P Salunkhe and SS Kadam
Abstract: The present study was carried out to asses the genetic charactorization among different varieties of finger millet by utilising 14 ISSR (dominant) and 6 SSR (co-dominant) markers. The DNA from leaf sample was isolated by standard protocol, while quite modifications in rapid protocol using 0.080 g ml-1 glucose, 0.020 g ml-1polyvinylpyrrolidone and 0.0050 g ml-1 sodium dodecyl sulphate produced the best quantity DNA. The 14 ISSR and 6 SSR pattern of genomic DNA of 14 varieties were analyzed with respect to the fragments by standard PCR components. During ISSR assay, total of 795 scorable DNA fragments were produced and among them 235 DNA fragments were found to be polymorphic, i.e. 29.74 % of polymorphism. SSR assay yields total of 109 scorable DNA fragments were produced and among them 95 DNA fragments were found to be polymorphic i.e. 83.33% polymorphism. In ISSR assay, the primer UBC-812 (0.833) and SSR primer UGEP 12 (0.5) and UGEP 67 (0.5) produced maximum polymorphic information content. The similarity co-efficient ranged from 0.719 (between varieties D-2 and SCN-10) to 0.982 (between varieties SCN-14 and M-1) in ISSR and 0.444 (between genotype SCN-6 and M-3) to 0.889 (between genotype Dapoli-2, Dapoli-1, SCN-4, SCN-8, SCN-10, SCN-11, SCN-13, SCN-14, Dapoli-safed in SSR indicated the distinctness of these varieties. Hence, the study indicated the more efficiency of SSR than ISSR markers in investigating genetic variability at molecular level.
DOI: 10.22271/chemi.2020.v8.i6v.10984
Pages: 1544-1549 | 394 Views 81 Downloads
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How to cite this article:
S Yadav, P Salunkhe, SS Kadam. Assessment of genetic relationship among varieties of finger millet using ISSR and SSR markers. Int J Chem Stud 2020;8(6):1544-1549. DOI: 10.22271/chemi.2020.v8.i6v.10984
