Vol. 9, Issue 2 (2021)
GC-MS analysis of bioactive components and evaluation of In-vitro pancreatic lipase inhibitory activity of aqueous extracts of Pleurotus eryngii
Author(s): Keshamma Entooru, Krishnaprasad Musalappa Srinivasalu, Sridhar Bilagumba Thimmaiah, Haleshappa Rangappa, Shivakumara Kanchidoddi Nanjaiah, Rajeev Ramachandra Kolgi, Roy Uddapanda Bopaiah
Abstract: Present study was designed to conduct with main purpose to determine bioactive components and evaluation of aqueous extract of Pleurotus eryngii for in-vitro pancreatic lipase inhibitory activity. GC-MS analysis was carried out to determine the bioactive components and in-vitro pancreatic lipase inhibitory assay was carried out to determine IC50 values of aqueous extracts of Pleurotus eryngii. The results of the present study depicted that the aqueous extracts of Pleurotus eryngii possess in-vitro pancreatic lipase inhibitory activities at the concentration of 1-30 µg/ml and this could be attributed to the prevailing compounds identified in the GC-MS analysis i.e., conhydrin, diethyl phthalate, phthalic acid-butyl hex-3-yl ester (alkaloids), ar-turmerone (sesquiterpenoid), palmitic acid, myristic acid, phenol and benzoic acid from ethanolic extract of Pleurotus eryngii. In conclusion, polyphenols, alkaloids terpenoids and Vitamin B class of secondary metabolites majorly identified in GC-MS analysis of aqueous extract of Pleurotus eryngii has been reported to possess the in-vitro pancreatic lipase inhibitory activities. Hence, further in-vivo studies in experimentally induced obese animal models could be recommended to access the safety and efficacy of aqueous extracts of Pleurotus eryngii to strongly recommend them as natural antiobesity agents in the formulations of natural antiobesity drugs.
Pages: 1141-1145 | 376 Views 67 Downloads
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How to cite this article:
Keshamma Entooru, Krishnaprasad Musalappa Srinivasalu, Sridhar Bilagumba Thimmaiah, Haleshappa Rangappa, Shivakumara Kanchidoddi Nanjaiah, Rajeev Ramachandra Kolgi, Roy Uddapanda Bopaiah. GC-MS analysis of bioactive components and evaluation of In-vitro pancreatic lipase inhibitory activity of aqueous extracts of Pleurotus eryngii. Int J Chem Stud 2021;9(2):1141-1145.
