Vol. 6, Issue 3 (2018)
Isolation and characterization of siderophore producing rhizobia from Sesbania sesban using different types of Indian soils
Author(s): Kuldeep Singh, Rajesh Gera and Rakesh Kumar
Abstract: In present study fourteen rhizobia were isolated from root nodules of Sesbania sesban by using trap plant method from diverse agroecological zones of Indian soils. These isolates produced round, whitish, smooth surface colonies on YEMA medium plates, short rod in shape, motile in nature and gram negative in reaction. All the rhizobial isolates were studied for its ability to produce siderophore on Chrome-Azurol S agar medium plates after 7th days of incubation at 28±2 oC. The results of these revealed that 43% of the rhizobial isolates were siderophore producers. Siderophore producing isolates were categorized as high, moderate and poor on the basis of yellow halo zones formation around the colonies, due to decolourization of the blue-coloured ferric dye complex spotted on the CAS agar medium plates. These results exposed the potential value of some isolates to be developed as bioinoculant for Sesbania sesban crop.
Pages: 797-800 | 550 Views 75 Downloads
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How to cite this article:
Kuldeep Singh, Rajesh Gera, Rakesh Kumar. Isolation and characterization of siderophore producing rhizobia from Sesbania sesban using different types of Indian soils. Int J Chem Stud 2018;6(3):797-800.
