Vol. 6, Issue 3 (2018)
Assessment of genetic diversity through SSR markers in Labeo rohita and Labeo gonius from two reservoirs of Uttarakhand
Author(s): Mohd Danish and IJ Singh
Abstract: The present study deals with the assessment of genetic diversity using microsatellite marker in the fish Labeo gonius from Nanak Sagar and Dhaura reservoirs of Uttarakhand having different morpho-edhaphic features and self- recruiting populations of this fish. These reservoirs are distantly located and distinctly separated without any connection having negligible possibility of gene exchange with each other. Total 12 microsatellite primers were selected and by using software Primer-BLAST and Primer-3 and all the designed microsatellite primers were screened in all 100 DNA samples of fish collected from both the reservoirs. Screened 12 microsatellite loci were successfully amplified. After PCR amplification of microsatellite loci and performing native PAGE using amplified DNA samples as above, POP GENE Version 1.32 was used to calculate Nei’s observed heterozygosity, expected heterozygosity, Nei’s genetic diversity, Fixation index (Fis) and Shannon’s information index (SI) and genetic variability indices viz. Gene flow(Nm), the coefficients of genetic differentiation ( Fst & Gst) and Nei’s genetic distance. Genetic diversity and level of heterozygosity in L. gonius were found to be maximum (Hi=0.6770 and Ho=0.5046)) from Nanak Sagar reservoir followed by genetic diversity in L.gonius from Dhaura reservoir (Hi=0.5732andHo=0.4901) and genetic diversity in L. rohita (Hi=0.561 and Ho=0.4894) from Nanak Sagar reservoir. L.rohita from Dhaura reservoir shows minimum genetic diversity value (Hi=0.4894and Ho=0.4226). Genetic diversity values are lower in Dhaura reservoir which might be due to its reduced effective population size associated with the small size of reservoir, extensive fishing pressure and drying up of major portion in summer season. These reservoirs are distantly located and have no connection with each other indicating negligible gene exchange with each other which is responsible for weak sub structuring of L.rohita and L.gonius stocks. Overall Gst value (0.1601) recorded for L. gonius suggested the possibility of less gene exchange among the two stocks and indicated that 16.01% variation was attributable to interstock divergence, while 83.99% to individual differences within the stocks.
Pages: 997-1001 | 626 Views 69 Downloads
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How to cite this article:
Mohd Danish, IJ Singh. Assessment of genetic diversity through SSR markers in Labeo rohita and Labeo gonius from two reservoirs of Uttarakhand. Int J Chem Stud 2018;6(3):997-1001.
