Vol. 6, Issue 3 (2018)
Clinico-patho-physiological changes associated with carbon tetrachloride (CCl4) induced sub-acute hepatotoxicity in rats
Author(s): Mritunjay Kumar, DB Mondal, Ajay Kumar and Monalisha Sahu
Abstract: CCl4 is widely used to induce hepatotoxicity in animal models although its mechanism of action in relation to changes associated with apoptosis, oxidative stress and APPs are not clearly understood. In present study sub acute hepatotoxicity was induced by CCl4 along with olive oil 1:1 v/v @ 2 ml/kg b wt intra-gastrically twice a week to evaluate changes associated with oxidative, histopathological, apoptotic and relative quantification of haptoglobin (Hp), C-reactive protein (CRP) and alanine aminotransferase (ALT) in liver tissue apart from biochemical changes rat serum. Eighteen randomly selected rats were divided into three groups consisting of healthy control (Gr I), disease control receiving CCl4 for 17 days (Gr II), and CCl4 receiving for 35 days (Gr III). Biochemically, Reduced values of serum total protein, albumin and globulin and increased level of serum ALT, AST, ALP, total and direct bilirubin and glucose were observed in disease control groups. However, decreased level of oxidative stress indices except LPO were noticed in rat liver tissue receiving CCl4. Histopathologically, hepatotoxic degenerative changes along with moderate fatty changes in the hepatocytes, mostly in the centrilobular region in Gr II while cytoplasmic vacuolations of variable size with marginal nucleus in hepatocytes and severe fat globules in Gr III were observed. Increased level of apoptotic and necrotic hepatocytes along with relatively increased level of Hp, CRP and ALT in rat liver tissue exposed to CCl4 till day 18 followed by remarkable decrease in relative level of Hp and CRP by day 36 of study.
Pages: 2670-2676 | 343 Views 42 Downloads
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How to cite this article:
Mritunjay Kumar, DB Mondal, Ajay Kumar, Monalisha Sahu. Clinico-patho-physiological changes associated with carbon tetrachloride (CCl4) induced sub-acute hepatotoxicity in rats. Int J Chem Stud 2018;6(3):2670-2676.
