Vol. 6, Issue 3 (2018)
Molecular detection of Salmonella enterica Subspecies enterica serotypes in foods of animal origin by PCR
Author(s): Surendra Singh Shekhawat, Abhishek Gaurav, Bincy Joseph, Shiv Kumar Sharma, Rohitash Dadhich and CS Vaishnava
Abstract: This study was aimed at identifying Salmonella enterica sub species enterica serotypes by amplification of salmonella genus specific 16S ribosomal RNA gene (16SrRNA) in foods of animal origin in and around Udaipur city. Food samples (total, n=632) of animal origin i.e. meat (n=206) milk (n=215) and egg (n=211) were purchased randomly from various retails shops located in the study area. Of the 632 samples, 10 isolates (1.58%) were positive for salmonella by conventional culture method specified by IS 5887 (Part 3):1999. DNA isolated from all the ten isolates was followed by molecular confirmation by polymerase chain reaction of 16SrRNA gene. The PCR products were subjected to agarose gel electrophoresis and all the ten isolates were found to be positive for 16SrRNA gene. This shows that PCR can be used as an alternative to the conventional methods of salmonella detection in food samples. The study showed that 16SrRNA primers are highly specific and sensitive for salmonella detection and the results can be obtained within 4-5 hours’ time. 16sRNA based PCR will be rapid and effective method for the surveillance of salmonella in foods of animal origin. Thus PCR offer a rapid and good diagnostic tool for routine monitoring of salmonellae in food samples compared to the conventional culture method.
Pages: 3586-3590 | 518 Views 104 Downloads
download (6157KB)
How to cite this article:
Surendra Singh Shekhawat, Abhishek Gaurav, Bincy Joseph, Shiv Kumar Sharma, Rohitash Dadhich, CS Vaishnava. Molecular detection of Salmonella enterica Subspecies enterica serotypes in foods of animal origin by PCR. Int J Chem Stud 2018;6(3):3586-3590.
