Vol. 6, Issue 4 (2018)
Effect of different auxins and cytokinins in callus induction and shoot regeneration in banana (Musa paradisiaca L.) variety Udhayam under in vitro condition
Author(s): Shardulya Shukla, Yogesh Prasad, Anil Kumar Yadav, Pushkar Dixit, Babloo Patel, Vikash Yadav
Abstract: The experiment was pursued in Tissue Culture Laboratory of Department of Horticulture in Sardar Vallabhbhai Patel University of Agriculture & Technology Meerut during 2016-17 on Udhayam variety of Banana. MS media were prepared. The minimum time of callus induction (24.41 days) was observed in treatment 2,4-D 4.00 mgl-1; while maximum (39.42 days) was noted under control. With the combination of BAP and Kinetin, the earliest shoot initiation (9.87 days) was noted under BAP 4.00 mgl-1 + Kinetin 2.00 mgl-1. The minimum days taken for shoot develop (11.47 days) was noted under BAP 4.00 mgl-1 + Kinetin 2.00 mgl-1. After, 40 and 60 days maximum number of shoots obtained (5.40) and (71.10) were noted under the same treatment of BAP 4.00 mgl-1 + Kinetin 2.00 mgl-1. Maximum percentage of developed shoots obtained (80.95%) was noted under the same treatment of BAP and Kinetin. Maximum shoot length obtained (4.26cm), (5.03cm) and (6.10cm) after 28, 35 and 42 days under the treatment of BAP 4.00 mgl-1 + Kinetin 2.00 mgl-1. Viewing above observations it is concluded that 2,4-D 4.00 mgl-1 showed better performance on accordance of callus induction while BAP 4.00 mgl-1 + Kinetin 2.00mgl-1 showed better performance on accordance of shoot regeneration.
Pages: 2041-2045 | 425 Views 90 Downloads
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How to cite this article:
Shardulya Shukla, Yogesh Prasad, Anil Kumar Yadav, Pushkar Dixit, Babloo Patel, Vikash Yadav. Effect of different auxins and cytokinins in callus induction and shoot regeneration in banana (Musa paradisiaca L.) variety Udhayam under in vitro condition. Int J Chem Stud 2018;6(4):2041-2045.
