Vol. 6, Issue 4 (2018)
Death kinetics of Bacillus subtilis spores during sterilization of aseptic packaging material by hydrogen peroxide
Author(s): Md. IA Ansari
Abstract: Aseptic packaging is a method of packaging in which pre-sterilized liquid food is packed and sealed in pre-sterilized container under aseptic conditions to produce shelf-stable products that do not need refrigeration. Sterilization of the packing material is a critical step before aseptic filling of liquid foods. Hydrogen peroxide was used for sterilization of packaging material. The death kinetics of Bacillus subtilis ATCC 6633 was studied during sterilization of packaging material. The initial count of spore taken for experimental purpose was 10 8 cfu / ml. In this study, inoculated packaging film (2x2cm2) was used for sterilization at hydrogen peroxide concentration of 10-30 % v/v, temperature of 50-80 °C and contact time of 30-90 second. The death kinetic parameters ‘k’, ‘D’ and Q10 were evaluated. It was found that reaction rate constant (k value) increases with rise in temperature while decimal reductio22n time (D value) decreases with rise in temperature at a given contact time and concentration of hydrogen peroxide. The temperature of hydrogen peroxide solutions had a pronounced effect on the rate of spore destruction. The estimated Q10 value was found be in the range of 1.00 to 2.1678828. The result could be used for designing sterilization process of food packaging material in aseptic packaging.
Pages: 2773-2777 | 409 Views 66 Downloads
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How to cite this article:
Md. IA Ansari. Death kinetics of Bacillus subtilis spores during sterilization of aseptic packaging material by hydrogen peroxide. Int J Chem Stud 2018;6(4):2773-2777.
