Vol. 6, Issue 5 (2018)
Effect of sterilization treatments on in vitro culture establishment of tomato (Solanum lycopersicum L.)
Author(s): Neha Bharti, Bhuvnesh Kapoor, Ishani Shaunak, Parul Sharma and Rajnish Sharma
Abstract: Sterilization of explants is a pre-requisite step for carrying out any plant tissue culture strategy. The type of chemicals used as fungicides viz. Carbendazim & Fenbendazole etc. and surface sterilants viz., sodium hypochlorite, calcium hypochlorite, hydrogen peroxide and mercuric chloride, etc. are being used in tissue culture to decontaminate the explants tissues. These sterilants have their adverse effect depending upon the type of explants, nature of sterilants and their specific duration when applied individually or in combination. In the present study, the effect of fungicide (Carbendazim) and surface sterilant (sodium hypochlorite) was documented on different type explants of tomato cv. Solan Lalima. During sterilization treatment, 0.2% Carbendazim for 10 min followed by different durations of 10% sodium hypochlorite viz., 8 min for true leaf (77.08%) and epicotyl (66.66%), 10 min for hypocotyl (74.30%), 6 min for cotyledon (61.80%) and 20 min for seeds (77.77%), respectively was found to be effective for getting maximum survival rate in vitro. Hence, the reported sterilization treatments could be useful for carrying out any biotechnological strategies such as in vitro cell line selection and development of transgenics against various biotic and abiotic stresses as well as for other qualitative parameters in tomato crop improvement programme.
Pages: 1165-1168 | 419 Views 60 Downloads
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How to cite this article:
Neha Bharti, Bhuvnesh Kapoor, Ishani Shaunak, Parul Sharma, Rajnish Sharma. Effect of sterilization treatments on in vitro culture establishment of tomato (Solanum lycopersicum L.). Int J Chem Stud 2018;6(5):1165-1168.
