Vol. 6, Issue 5 (2018)
Effect of sterilants on shoot regeneration of Banana (Musa acuminata) cv. Monthan in vitro plantlets
Author(s): Ankita Trivedi, RS Sengar, Ramji Singh, Bijendra Singh, SK Singh and Mukesh Kumar
Abstract: In vitro culture of plant species most commonly depends on the optimal size of explants used for the purpose of study. Relatively, larger explants (3-10 mm) are desirable for rapid multiplication despite of its higher susceptibility to blackening and contamination. The surface sterilization of banana shoot-tips explants of desired size was performed before transferring them to the culture media to make our cultures contamination free. In the present study, different sizes of explants and different sterilants were used for determining the growth parameters of cv. Monthan and to check their ability of making a media contamination free. The toxicity percentage was seen to be dependent on the sterilants concentration and duration of time for being they are used. Double disinfection method was also used in case of longer persistence of contamination.
Pages: 1915-1918 | 340 Views 60 Downloads
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How to cite this article:
Ankita Trivedi, RS Sengar, Ramji Singh, Bijendra Singh, SK Singh, Mukesh Kumar. Effect of sterilants on shoot regeneration of Banana (Musa acuminata) cv. Monthan in vitro plantlets. Int J Chem Stud 2018;6(5):1915-1918.
