Vol. 6, Issue 5 (2018)
Ornithine amino transferase (OAT) and δ1-pyrroline 5-carboxylic acid synthetase (P5CS) in context to 2-Acetyl-1-Pyrroline (2-AP) in the seedlings of fragrant and non fragrant rice genotype(s)
Author(s): Dinesh C Pathak, Mahesh Kumar Bharti, Deepika Chandra and AK Gaur
Abstract: 2-Acetyl-1-Pyrroline is one of the potent flavor compounds in rice aroma. Studies were carried out to find the candidate genes/enzymes involved in the 2-AP biosynthesis from proline, glutamate and ornithine precursors. Ornithine Amino Transferase (OAT) and δ1-Pyrroline 5-Carboxylic Acid Synthatase (P5CS) genes/enzymes were selected as the candidate genes/enzymes for the study in three rice genotypes Kalanamak 3119-2, Dehradun Basmati-3020 and Pant Dhan-4 (control). The specific enzyme activity pattern of OAT and P5CS was observed as Dehradun Basmati > Kalanamak > Pant Dhan-4. There was positive correlation between OAT and P5CS enzyme activity relative to 2-AP content. Genomic DNA of the three genotype(s) was subjected to PCR amplification using the gene specific primers of OAT and P5CS genes. Single band size of 604 bp was detected on the gel with OAT specific primers, while P5CS primers produced a 1671 bp band in all the three genotype(s). An additional band was also detected, whose size was slightly more than 604 bp when Dehradun Basmati-3020 genomic DNA was amplified using the OAT gene specific primers. Presence of two bands indicates that the genotype Dehradun Basmati-3020 may be containing more than one form of gene.
Pages: 2348-2352 | 364 Views 56 Downloads
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How to cite this article:
Dinesh C Pathak, Mahesh Kumar Bharti, Deepika Chandra, AK Gaur. Ornithine amino transferase (OAT) and δ1-pyrroline 5-carboxylic acid synthetase (P5CS) in context to 2-Acetyl-1-Pyrroline (2-AP) in the seedlings of fragrant and non fragrant rice genotype(s). Int J Chem Stud 2018;6(5):2348-2352.
