Vol. 6, Issue 5 (2018)
Genetic diversity analysis of garlic (Allium sativum L.) genotypes using ISSR markers
Author(s): Virendra Singh Shekhawat, RS Tomar, Rathva Sahadev R, Nikita Patel S and Ramesh Kumar
Abstract: A total of 10 ISSR primers were used among fifty five genotypes of garlic out of which 9 primers gave amplification and generated 35 fragments. The numbers of polymorphic bands were 32 having 31 shared and one unique band with an average of 3.56 bands and 90.37% polymorphism per primer. The PIC values for ISSR marker were ranged from 0.43 (UBC-836) to 0.8 (UBC-849) with an average value of 0.56 per primer and ISSR primer index (IPI) differed from 0.96 (UBC-825) to 4.00 (UBC-849) with an average value of 2.28 per primer. The dendogram generated by ISSR markers revealed two major groups, Cluster-I and cluster-II. Genotypes RGP 429, RGP 117, RGP 445, RGP 495 and RGP 609 showed maximum similarity in cluster-I, whereas RGP 235 and G 282 showed maximum similarity in cluster-II. Genotype GAG-6 and RGP-26 were found as most diverse genotypes among the genotypes.
Pages: 2394-2399 | 336 Views 67 Downloads
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How to cite this article:
Virendra Singh Shekhawat, RS Tomar, Rathva Sahadev R, Nikita Patel S, Ramesh Kumar. Genetic diversity analysis of garlic (Allium sativum L.) genotypes using ISSR markers. Int J Chem Stud 2018;6(5):2394-2399.
