Vol. 5, Issue 6 (2017)
UV-VIS spectrophotometric determination of Nickel (II) using 3, 5-dimethoxy-4-hydroxy benzaldehyde isonicotinoyl hydrazone (DMHBIH) from polluted water of Bagmati River, Kathmandu, Nepal
Author(s): Surendra Prasad Singh
Abstract: The Bagmati River is being polluted excessively in such a manner that if the trend continues then obviously the Bagmati will be dead for all phenomenon. Similarly Bagmati is also more or less contaminated with the heavy metals such nickel, lead, mercury, cadmium and chromium. The contamination may be due to the waste disposed near the river side, sewerage discharge and industrial waste water effluents and river bank encroachment. Nickel (II) is a metal ion appearing together in a wide variety of samples of the food industry, in the manufacture of alloys, the production of heat-resistant steels and is present in small amounts in most soils, plants and in drainage water. Colorimetry is defined as the method of trace analysis that involves absorption of radiations having frequencies in the visible spectrum. This is done using a Spectrophotometer that transmits radiation from a source through a sample cell and into a detector which measures the absorbance of the sample. The wavelength of radiation absorbed depends on the identity and amount of substance present in the sample. The relationship between these factors is called the Beer-Lambert law, the Bouger-Beer law or more simply, Beer’s law. Thus, in this experiment, UV-Vis Spectrophotometeric determination of Nickel (II) with 3, 5-Dimethoxy-4-hydroxybenzaldehyde isonicotinoyl hydrazone (DMHBIH) reagent was proposed in basic buffer solution (pH 9.0). Samples were collected from Bagmati river of Kathmandu and the maximum absorbance was observed in the pH range 8.5-9.5. The molar absorptivity and Sandell’s sensitivity of Nickel (II) complex with DMHBIH at λ max 386 nm was found to be 1.82 x 104 L mol-1cm-1 and 0.0082 µg/cm2. Beer’s law validity range varies from 0.234 to 2.94 µg /ml. Nickel (II) forms 1:1 complex with DMHBIH and stability constant of Nickel (II) complex was 9.4X106. The first order derivative amplitude was measured by the peak height method at λ max 440 nm. The second order derivative amplitude was measured by the peak height method at λ max 470 nm. The developed spectrophotometric method was applied for the determination of Nickel (II) in alloy samples.
Pages: 2187-2189 | 870 Views 368 Downloads
download (5048KB)
How to cite this article:
Surendra Prasad Singh. UV-VIS spectrophotometric determination of Nickel (II) using 3, 5-dimethoxy-4-hydroxy benzaldehyde isonicotinoyl hydrazone (DMHBIH) from polluted water of Bagmati River, Kathmandu, Nepal. Int J Chem Stud 2017;5(6):2187-2189.
