Vol. 6, Issue 6 (2018)
Purification and characterization of Alkaline Phosphatase from Pseudomonas aeruginosa isolated from apple rhizosphere
Author(s): Ranjna Sharma, Rajni Kant Thakur and Mohinder Kaur
Abstract: An extracellular alkaline phosphatase was purified from potential phosphate solubilizing strain of Pseudomonas aeruginosa An-H isolated from rhizospheric soil of apple. Alkaline phosphatase was purified using ammonium sulphate precipitation and Sephadex G-100 gel filtration chromatography with specific activity of 1.93 U/mg of protein in crude extract and specific activity reached to 5.63 U/mg of protein in ammonium sulphate precipitation. On Sephadex G-100 column chromatography, specific activity reached to 30.29U/mg of protein with purification fold of 15.69. The molecular weight of purified Pseudomonas aeruginosa An-H was recorded as 66 kDa on 10% SDS gel electrophoresis. The pH optima of this enzyme is 9.0 in 0.1 M Tris-HCl buffer with optimum temperature of 47oC. Production and purification of this enzyme in NBRIP medium shows that the Pseudomonas aeruginosa An-H solubilize the phosphate associated with calcium ions and this available phosphorus is easily used by apple plants for their growth and development. Therefore, the aim of the study was to check the production and purification of alkaline phosphatase.
Pages: 1156-1159 | 451 Views 158 Downloads
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How to cite this article:
Ranjna Sharma, Rajni Kant Thakur, Mohinder Kaur. Purification and characterization of Alkaline Phosphatase from Pseudomonas aeruginosa isolated from apple rhizosphere. Int J Chem Stud 2018;6(6):1156-1159.
