Vol. 6, Issue 6 (2018)
Selection of F2 plants through hydroponics screening at higher iron toxic levels for bulk segregant analysis (BSA) in rice
Author(s): M Amaranatha Reddy, Rose Mary Francies, Jiji Joseph, CR Elsy and PS Abida
Abstract: Phenotypic screening of 300 F2 plants at higher toxic iron levels of hydroponics revealed that wide variability is observed for shoot length, root length, total number of roots, number of fresh roots, shoot weight, root weight and visual scoring for iron-toxicity symptoms. A total of 15 F2 plants and 87 F2 plants out of 300 plants had exhibited leaf bronzing score 1 and 9 respectively at six weeks after exposure to iron stress at 800ppm of Fe. Ten most tolerant and ten most susceptible plants selected from 15 and 87 F2 plants respectively based on ranking of total score which is obtained by adding individual ranks of all characters of particular plant. Plant number 248, 320, 309, 111, 156, 20, 287, 268, 52 and 300 respectively from 15 F2 plants with highest overall score were selected as ten most tolerant plants. The selected susceptible plants with the least overall score were plant number 12, 202, 66, 18, 109, 113, 122, 334, 231 and 213 respectively. Resistant bulk DNA sample and Susceptible DNA sample was prepared by mixing equal amounts of DNA at same concentration from ten most tolerant F2 plants and ten most susceptible F2 plants respectively.
Pages: 2217-2223 | 1472 Views 1238 Downloads
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How to cite this article:
M Amaranatha Reddy, Rose Mary Francies, Jiji Joseph, CR Elsy, PS Abida. Selection of F2 plants through hydroponics screening at higher iron toxic levels for bulk segregant analysis (BSA) in rice. Int J Chem Stud 2018;6(6):2217-2223.
