Vol. 6, Issue 6 (2018)
Influence of sodium nitroprusside on sperm functional membrane integrity and acrosome integrity of frozen thawed buffalo semen
Author(s): Domple Vijay Digamber, K Loganathasamy, V Leela and P Kumarasamy
Abstract: The present study was undertaken to assess the effects of exogenous supplementation of sodium nitroprusside (SNP), a nitric oxide (NO) donor on sperm functional membrane integrity and acrosome integrity of frozen thawed buffalo semen. Frozen semen straws from 6 buffalo bulls were procured from Central Frozen Semen Production and Training Institute, Hesseraghatta, Banglore-51. The straws were thawed at 37˚C for 30 seconds and emptied into 15 ml sterile plastic centrifuge tube containing 1 ml of capacitation medium (control), addition of 100 µM SNP to capacitation medium (SNP treatment I) and 100 nM SNP to capacitation medium (SNP treatment II) were incubated at 37˚C for 1 hour. After 1 hour incubation, sperm functional membrane integrity was studied by hypo-osmotic swelling test (HOST). In control, SNP treatment I and II, 57.42% ± 0.62, 28.08% ± 1.02 and 39.75% ± 1.20 spermatozoa had intact functional membrane. Functional membrane integrity of spermatozoa was significantly (P<0.01) higher in control than SNP treatment I and II. Spermatozoa with intact functional membrane were significantly (P<0.01)more in SNP treatment II than SNP treatment I. Sperm acrosome integrity was assessed by Giemsa stain method. Acrosome intact spermatozoa were significantly (P<0.01) more in control (37.42% ± 0.70) as compared to SNP treatment I (12.58% ± 0.68) and II (27.33% ± 0.64). Spermatozoa with intact acrosome were significantly (P<0.01) high in SNP treatment II than SNP treatment I. From this study, it is concluded that addition of SNP, a NO donor has detrimental effects on sperm functional membrane integrity and acrosome integrity of frozen thawed buffalo semen on concentration dependant manner.
Pages: 2460-2463 | 298 Views 49 Downloads
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How to cite this article:
Domple Vijay Digamber, K Loganathasamy, V Leela, P Kumarasamy. Influence of sodium nitroprusside on sperm functional membrane integrity and acrosome integrity of frozen thawed buffalo semen. Int J Chem Stud 2018;6(6):2460-2463.
