Vol. 6, Issue 6 (2018)
Effect of sodium nitroprusside on sperm lipid peroxidation status of frozen thawed buffalo semen
Author(s): Domple Vijay Digamber, K Loganathasamy, V Leela and P Kumarasamy
Abstract: The experiment was conducted to assess the effect of exogenous supplementation of sodium nitroprusside (SNP), a nitric oxide (NO) donor on lipid peroxidation (LPO) status of frozen thawed buffalo semen. Frozen semen straws from 6 buffalo bulls were procured from Central Frozen Semen Production and Training Institute, Hesseraghatta, Banglore-51. The straws were thawed at 37 ˚C for 30 seconds and emptied into 15 ml sterile plastic centrifuge tube containing 1 ml of capacitation medium (control), addition of 100 µM SNP to capacitation medium (SNP treatment I) and 100 nM SNP to capacitation medium (SNP treatment II) and incubated at 37 ˚C for 1 hour. After incubation, sperm LPO status was measured by production of malondialdehyde (MDA) level. The MDA levels were significantly (P<0.01) low in control (1.48 µmol/ml ± 0.12) when compared to SNP treatment I (3.79 µmol/ml ± 0.07) and II (2.76 µmol/ml ± 0.06). Between treatments, MDA level was low in SNP treatment II than SNP treatment I. This study indicates that addition of SNP in the capacitation medium increases sperm LPO in frozen thawed buffalo semen in concentration dependent manner.
Pages: 2918-2920 | 327 Views 39 Downloads
download (4487KB)
How to cite this article:
Domple Vijay Digamber, K Loganathasamy, V Leela, P Kumarasamy. Effect of sodium nitroprusside on sperm lipid peroxidation status of frozen thawed buffalo semen. Int J Chem Stud 2018;6(6):2918-2920.
