Vol. 7, Issue 1 (2019)
Standardization of protocol for sterilization and in vitro regeneration in tuberose (Polianthes tuberosa L.)
Author(s): K Khanchana, Dr. M Kannan, Dr. K Hemaprabha and Dr. M Ganga
Abstract: Investigations on micro-propagation of four tuberose varieties viz., Arka Nirantara, Shringar, Calcutta Double and Vaibhav were carried out at the Tissue Culture laboratory of the Horticultural College and Research Institute of TNAU, Coimbatore during 2014-2016. Two types of explants namely axillary bud and bulblets were used. Of these, axillary buds followed by bulblets were found to be the most suitable explants with respect to response for in vitro regeneration and multiplication. The best treatment for sterilization was treating the explants with 0.5% Bavistin solution along with teepol for two hours and 5% NaOCl for 10 minutes and for shoot regeneration and elongation are full strength MS medium fortified with BAP (2 mg/l) + NAA (0.5 mg/l). Efficient rooting was achieved with ½ MS medium fortified with 1 mg/l of IBA + 1 mg/l of NAA for all the four varieties. Rooted plantlets were hardened with pot mixture (1:1:1) which resulted in the highest rate of ex vitro survival. Experiments taken up to standardize methods to rescue microbial contaminated cultures indicated that slicing off the infected portion and treating with Bavistin was found to be the best for controlling fungal contamination, whereas, adding the antibiotic streptomycin in the medium was the best, for controlling bacterial contamination.
Fig. 1: Different developmental stages in micro propagation of axillary bud
Fig. 2: Different developmental stages in micro propagation of bulblets
Pages: 236-241 | 1114 Views 288 Downloads
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How to cite this article:
K Khanchana, Dr. M Kannan, Dr. K Hemaprabha, Dr. M Ganga. Standardization of protocol for sterilization and in vitro regeneration in tuberose (Polianthes tuberosa L.). Int J Chem Stud 2019;7(1):236-241.
