International Journal of Chemical Studies
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P-ISSN: 2349-8528, E-ISSN: 2321-4902   |   Impact Factor: GIF: 0.565

Vol. 7, Issue 1 (2019)

Blood biochemical indices lack diagnostic significance in subclinical caprine brucellosis: Evidence from non-vaccinated Brucella melitensis positive Changra goats in Northern Himalayan region of Ladakh


Rose Bengal Plate Agglutination test (RBPT): Note the clear clump formation depicting agglutination reaction
Fig. 1: Rose Bengal Plate Agglutination test (RBPT): Note the clear clump formation depicting agglutination reaction


Standard Tube Agglutination Test (STAT):Tubes 1 to 6: dilution1:10, 1:20, 1:40, 1:80, 1:160 & 1:320; Tube C: Control; Note the mat formation upto tube-4 (1:80 dilution), turbidity in tubes 5,6 and control.
Fig. 2: Standard Tube Agglutination Test (STAT):Tubes 1 to 6: dilution1:10, 1:20, 1:40, 1:80, 1:160 & 1:320; Tube C: Control; Note the mat formation upto tube-4 (1:80 dilution), turbidity in tubes 5,6 and control.


Dot-ELISA for <em>Brucella melitensis</em>: Note blue dot formation in positive samples
Fig. 3: Dot-ELISA for Brucella melitensis: Note blue dot formation in positive samples


PCR amplification of <em>Brucella</em> <em>melitensis</em> specific IS711 (252bp): M: Molecular weight marker (100bp+); P: Positive control; N: Negative Contro; 1&2: Positive samples
Fig. 4: PCR amplification of Brucella melitensis specific IS711 (252bp): M: Molecular weight marker (100bp+); P: Positive control; N: Negative Contro; 1&2: Positive samples


 

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International Journal of Chemical Studies