Vol. 7, Issue 1 (2019)
Molecular characterization and DNA fingerprinting of Centella asiatica using SSR markers
Author(s): Rohini MR, Anuradha Sane, Ravish Chaudhary and Himabindu K
Abstract: Centella asiatica (L.) Urban is an important medicinal herb used both in the traditional and modern system of medicine. Owing to its growing demand, consumption by Indian herbal industry is increasing and has got great export demand. Being a clonally propagated species, it is very difficult to differentiate the genotypes morphologically and to identify the superior cultivars. Thus, the present study was carried out to genetically characterize and develop DNA fingerprint of seventeen genotypes using SSR markers. Results showed that selected SSR markers could distinguish the collections and provide clonal identity. Both, normal and fluorescence based PCR technique was employed. The total number of alleles detected ranged from 1 to 8 with an average of 3.100 alleles per locus. The polymorphism percentage ranged from 85.71 to 100 with an average of 92.96% polymorphism across all the 17 accessions. The mean expected heterozygosity obtained was 0.456 and average PIC value of the marker obtained was 0.390. This study enabled to develop DNA barcode of the studied genotypes which would further help in establishing clonal identity, avoiding duplicate accessions, maintaining purity of elite lines and assuring quality of raw drug.
Fig. 1: C. asiatica genotypes used in the study
Fig. 2: 3-D plot derived based on PCA analysis of molecular data of 17 accessions of C. asiatica using SSR markers
Fig. 3: UPGMA Dendrogram generated based on SSR data of 17 C. asiatica accessions
Pages: 705-710 | 590 Views 131 Downloads
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How to cite this article:
Rohini MR, Anuradha Sane, Ravish Chaudhary, Himabindu K. Molecular characterization and DNA fingerprinting of Centella asiatica using SSR markers. Int J Chem Stud 2019;7(1):705-710.
