Vol. 7, Issue 1 (2019)
Biochemical and physiological characterizations of Pseudomonas fluorescens
Author(s): Labhasetwar AA, SB Bramhankar, TS Pillai, SS Isokar, GT Dinkwar, SS Bhure and VM Kharat
Abstract: Seven Pseudomonas isolates obtained from rhizosphere of different crops were designed as PF-1 to PF-7 which showed the characteristics of Pseudomonas. All the isolates were gram negative, rod shaped and produced yellow and greenish yellow pigment. Biochemical characterizations were studied viz., KOH test, catalase test, starch hydrolysis, gelatin liquefaction, H2S production, acid and gas production, some of the isolates show both positive and negative to starch hydrolysis, H2S production, IAA production and phosphate solubilization. The bacterium failed to produce hydrogen cyanide (HCN). Physiological studies revealed that all isolates show maximum growth at 25 and 30°C temperature with OD value ≥ 60. All the isolates show maximum growth at pH 7 and 8 with OD vale ≥ 60. Antagonistic activity of Pseudomonas fluorescens isolates were studied against Xanthomonas axonopodis pv. citri. There were significant differences on inhibition zone. Isolate PF-7 recorded maximum zone of inhibition (29.1 mm) followed by PF-4 (28.7 mm), whereas no inhibition showed by isolate PF-6.
Pages: 1785-1788 | 876 Views 586 Downloads
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How to cite this article:
Labhasetwar AA, SB Bramhankar, TS Pillai, SS Isokar, GT Dinkwar, SS Bhure, VM Kharat. Biochemical and physiological characterizations of Pseudomonas fluorescens. Int J Chem Stud 2019;7(1):1785-1788.
