Vol. 7, Issue 2 (2019)
Genetic diversity of wood apple (Feronia limonia L.) revealed by random amplified polymorphic DNA
Author(s): R Bhargava, Karun Gurjar, AK Singh and Sanjay Singh
Abstract: Wood Apple (Feronia limonia) belongs to the family Rutaceae. It is also known as monkey fruit, curd fruit, kotha, Kavat and kathbel. The different parts of plant (leaf, stem, bark, fruit, and seed) have been used for curing various diseases. The fruit is used for curative properties, which make the tree one of the useful medicinal plants of India. It is subtropical and grows in abundance throughout India’s drier regions. Due to lack of awareness regarding its significance with respect to their nutritional and therapeutical values there is no systematic cultivation of wood apple in India is not available. A wide genetic diversity in wood apple in terms of fruit shape, size, colour and qualitative characters are found in wild, roadside, in farmhouse garden, and it is still under unexploited condition. Considering the wide gene diversity in wood apple, ten accessions have been collected from Central Horticultural Experiment Station (CIAH), Vejalpur, Panchmahals (Godhra), Gujarat to assess the genetic diversity in wood apple using Random Amplified Polymorphic DNA (RAPD) technique. Twenty five arbitrary random primers were used to determine RAPD polymorphism. A total of 192 amplified bands were scored from the 22 RAPD primers, out of those 139 polymorphic bands (PPB) was found. High rate of polymorphism was observed reasonably for RPID-5, RPID-16, RPID-23, RPID-24 and RPID-25 primers. The size of PCR products ranged from 250 bp to 4500 bp. The number of amplified DNA bands varied between 2 (primer RPID-13) and 16 (primer RPID-24 and RPID-25). The highest genetic similarity value coefficient was observed between genotypes CHESW12 and CHESW4 was 0.915 where as the minimum genetic similarity coefficient was observed between genotypes genotype CHESW9 and CHESW10. The dendrogram based on hierarchical cluster analysis using UPGMA algorithm was prepared. The cultivars were grouped into two main clusters. RAPD profiles developed using primers RPID-3, RPID-4, RPID-6, RPID-9, RPID-10, RPID-11, RPID-16, RPID-18, RPID-19, RPID-20, RPID-21, RPID-23, RPID-25 showed the typical/specific band combinations which can be useful for identification of genotype. In this experiment, RAPD proved to be a rapid, reliable and practicable method for revealing of polymorphism in the wood apple genotypes.
Fig. 1: The location of the wood apple genotypes sample used in the study.
Fig. 2: Genetic polymorphism among wood apple genotypes as revealed by RAPD analysis. M: 1 kbp DNA ladder.
Fig. 3: Clustering of 11 wood apple genotypes based on RAPD profiling.
Pages: 208-212 | 711 Views 100 Downloads
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How to cite this article:
R Bhargava, Karun Gurjar, AK Singh, Sanjay Singh. Genetic diversity of wood apple (Feronia limonia L.) revealed by random amplified polymorphic DNA. Int J Chem Stud 2019;7(2):208-212.
