Vol. 7, Issue 2 (2019)
Genetic variability in Albugo candida pathogen isolates collected from Indian mustard in Northern Madhya Pradesh using RAPD marker analysis
Author(s): Ravi Yadav, RK Pandya, Sushma Tiwari, MK Tripathi and Bhagyashee Singh
Abstract: Rapeseed-mustard group of crops are moist important rabi oilseed crops cultivated in Northern region of Madhya Pradesh. Indian mustard [Brassica juncea (L.) Czern and Coss] is the dominating crop occupying >80% share of the total area of rapeseed-mustard in the region. White rust incited by Albugo candida ((Pers. ex. Lev.) Kuntze) is one of the major biotic constraints of Indian mustard in the state. The white rust infected leaf samples were collected from five major mustard growing districts of Madhya Pradesh viz., Morena, Bhind, Sheopur, Gwalior and Datiya to find out the genetic variability occurring in the pathogen across the region. To show genetic distance among fungal isolates, banding pattern of RAPD marker was used to construct phylogenetic tree by distance matrix analysis. The phylogenetic tree was clearly showed genetic variation in different isolates Genetic variability showed by five isolated of Albugo candida illustrated in dendrogram, framing three major cluster, in which Isolate L5 was completely different from rest of the isolates and made separate cluster. All other samples were also significantly different from each other i.e. L4(25%),L2(35%) and L1 & L3(67%).
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Fig. 1: .
Fig. 2: Genomic DNA concentration of five different leaf samples (L1; 2, L2; 3, L3; 4, L4; 5,)
Fig. 3: Gel pictures showing RAPD profile of five samples with Primer 1(A), Primer 2 (B) and Primer 3 (c) (L1: 500 bp ladder, 1: sample from L1, 2: Sample from L2, 3: Sample from L3, 4: Sample from L4, 5: Sample from L5)
Pages: 237-241 | 548 Views 61 Downloads
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How to cite this article:
Ravi Yadav, RK Pandya, Sushma Tiwari, MK Tripathi, Bhagyashee Singh. Genetic variability in Albugo candida pathogen isolates collected from Indian mustard in Northern Madhya Pradesh using RAPD marker analysis. Int J Chem Stud 2019;7(2):237-241.
