Vol. 7, Issue 2 (2019)
Effect of plant growth regulators on high frequency callus initiation in vilvam (Aegle marmelos Corr.), a sacred tree species
Author(s): Akshara B, Renuka R, Santhanakrishnan VP, Kavino M and Rajesh S
Abstract: Aegle marmelos (L.) Corr. commonly known as “Bael†is mostly used in Ayurveda, Unani and other traditional systems of medicine for treating various human ailments. Owing to its high therapeutic value, bael is being overexploited for the extraction of secondary metabolites. As a prerequisite for the establishment of cell suspension cultures and in vitro regeneration of plants, an efficient protocol for the callus induction was developed using different explants with varied hormonal concentrations of auxins (NAA, 2, 4-D) and cytokinins (BAP,KIN) either alone or in combinations. Murashige and Skoog (MS) medium supplemented with 3.5 mg L-12, 4- D alone exhibited highest callusing frequency (100%) in hypocotyl explants from in vitro germinated seedlings followed by 97.2% in de-embryonated cotyledon in MS media with 3.0 mg L-1 2, 4-D.
Pages: 726-731 | 388 Views 56 Downloads
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How to cite this article:
Akshara B, Renuka R, Santhanakrishnan VP, Kavino M, Rajesh S. Effect of plant growth regulators on high frequency callus initiation in vilvam (Aegle marmelos Corr.), a sacred tree species. Int J Chem Stud 2019;7(2):726-731.
