Vol. 7, Issue 2 (2019)
In vitro propagation of crossandra (Crossandra infundibuliformis (L.) Nees.) var. Arka Shravya and Pondicherry local through direct organogenesis
Author(s): Kalaivani K, Rajadurai KR, Hemaprabha K and Kannan M
Abstract: Investigation on “In vitro propagation of crossandra (Crossandra infundibuliformis (L.) Nees.) var. Arka Shravya and Pondicherry local through direct organogenesis†was carried out at the Tissue Culture laboratory of the Horticultural College and Research Institute of TNAU, Coimbatore. In both the varieties shoot tips and nodal segments were used as explants. Of these, nodal segments were found to be the most suitable explants with respect to response for in vitro regeneration and multiplication. Nodal segments pre-treated with 0.2% Bavistin (30 min.) + 0.5% Streptomycin (45 min.) + 70% Ethanol (30 sec.) + 0.5% NaOCl (5 min.) registered the highest survival per cent. Shoot tips pre-treated with 0.2% Bavistin (30 min.) + 0.3% Streptomycin (45 min.) + 70% Ethanol (30 sec.) + 0.5% NaOCl (5 min.) registered the highest survival per cent. MS medium + BAP (2 mg l-1) was found to be best for nodal segment cultures with respect to the maximum percentage response to shoot proliferation, days required for shoot emergence and number of shoots per explant. MS medium + BAP (2 mg l-1) + GA3 (1 mg l-1) was found to be best with response to highest shoot elongation percentage of shoots and average length of shoots. Efficient rooting was achieved with ½ MS medium + IBA (1 mg l-1), gave the maximum in vitro rooting percentage. Among the two varieties compared in crossandra, Arka Shravya proved to be more potential than Pondicherry Local, in respect of time taken for in vitro regeneration and rooting.
Pages: 1606-1609 | 389 Views 71 Downloads
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How to cite this article:
Kalaivani K, Rajadurai KR, Hemaprabha K, Kannan M. In vitro propagation of crossandra (Crossandra infundibuliformis (L.) Nees.) var. Arka Shravya and Pondicherry local through direct organogenesis. Int J Chem Stud 2019;7(2):1606-1609.
