Vol. 7, Issue 2 (2019)
Assessment of genetic diversity in advance breeding lines of Chilli (Capsicum annuum L.) using RAPD and Cytochrome P450 gene based marker system
Author(s): Rinchan Dolkar, Pavan Kumar, Manjunatha Girigowda and Pallavi HM
Abstract: Chilli (Capsicum annuum L.) is regarded as one of the major commercial spice crops. India is the largest producer of Chilli in the world. The present work Genetic relationship between sixteen advance breeding lines was analyzed using randomly amplified polymorphic DNA and Cytochrome P450 gene based markers. A total of 35 RAPD and 11 Cytochrome P450 gene based markers analysis generated an average of polymorphism of 37.625 % and 29.17 % respectively. Multiplex ratio (MR) was recorded highest for cytochrome P450 gene based markers (10.18) compared to RAPD (6.34). Based on the marker data, 16 breedling lines of Chilli were grouped into two main and two sub-clusters, without any distinct pattern. High bootstrap value obtained at major nodes indicates robustness of the dendrogram. Pearson’s coefficient was determined by mantle test to establish cophenetic correlation with the value of 0.884 from both the marker system. The dendrogram and PCA plots obtained were found to be highly concordant to each other. Current research signifies that combination of these functional RAPD and non-functional cytochrome P450 gene based marker systems can be effectively utilized for understanding genetic variability between advance breeding lines, which can be utilize for breeding strategies and development of improved verities.
Pages: 1656-1663 | 470 Views 54 Downloads
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How to cite this article:
Rinchan Dolkar, Pavan Kumar, Manjunatha Girigowda, Pallavi HM. Assessment of genetic diversity in advance breeding lines of Chilli (Capsicum annuum L.) using RAPD and Cytochrome P450 gene based marker system. Int J Chem Stud 2019;7(2):1656-1663.
