Vol. 7, Issue 2 (2019)
Isolation and pathogenicity of pathogen causing diseases on dodder (Cuscuta gronovii)
Author(s): VR Bangar, MS Joshi and HT Valvi
Abstract: The disease symptoms on Cuscuta vine were observed in severe form in the survey for host range of Cuscuta in different locations of Raigad and Thane districts of Konkan region during 2015-16. The Cuscuta vine was found to be infected by different pathogens; symptoms appeared as discoloration and shrivelling of vine, blighted portion, necrotic lesions and tip necrosis on vine. The infected samples of Cuscuta were collected and brought to the laboratory for further studies. Under microscopic examination there are three mounts were placed. In that different morphological structure were observed. Different pathogens were isolated from diseased Cuscuta samples on PDA medium. After isolation of diseased samples there are three isolates were obtained. There colony and morphological characters are different from each other. Pathogenicity of the test fungi was proved by spraying with the spore suspension of individual fungus (105 spores/ml) on C. gronovii parasitizing on green gram plants (two weeks old) already grown in plastic pots. Symptoms appeared on the artificially inoculated C. gronovii vine within period of 7 to 14 days after inoculation. Three fungi viz., Fusarium incarnatum (Desm.) Sacc, Alternaria dianthicola Neerg and Curvularia pallescens Boedijn were isolated from diseased Cuscuta samples on poatato dextrose agar medium (PDA). Further these three isolated fungi were confirmed as by the chief Mycologist, Agharkar Research Institute, Pune.
Pages: 1769-1773 | 545 Views 233 Downloads
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How to cite this article:
VR Bangar, MS Joshi, HT Valvi. Isolation and pathogenicity of pathogen causing diseases on dodder (Cuscuta gronovii). Int J Chem Stud 2019;7(2):1769-1773.
