Vol. 7, Issue 4 (2019)
Assessment of genetic diversity by using RAPD and ISSR markers in ginger (Zingiber officinale Rosc.) genotypes
Author(s): Virali Antala, Subhash Narayanan, Nidhi Radadiya, Hemangini Chaudhari, Hiral Desai and Ghanshyam Patil
Abstract: Ginger (Zingiber officinale Rosc.) is an herbaceous perennial, the rhizomes of which are used as a spice. Several promising cultivars released in ginger are characterized on the basis of morphological and biochemical data, oleoresin content and yield potential. However these characters differ under varying environmental conditions making characterization of different ginger cultivars a complicated task. In addition to this practical concern the continued release of new cultivars makes the development of new techniques for genetic purity determination even more essential. Due to resurgence of interest in the commercial development of different cultivars of ginger as new spice crops, it has become necessary to precisely characterize the genetic diversity that exists in cultivars, advanced selections and native population. This is one step towards providing accurate genetic information for future breeding and germplasm collection efforts. The present study was carried out with an aim to characterize 31 different ginger genotypes using molecular markers viz., RAPD and ISSR. RAPD amplification of genomic DNA using 34 primers amplified total 9210 scorable bands with 475 loci, among them, 370 loci were found polymorphic, showing 76.83% polymorphism. The PIC values ranged from 0.74 (OPE- 09) to 0.93 (OPG-07, OPG-09 and OPH-12) with an average of 0.88. The highest (100%) polymorphism was exhibited by primers viz., OPA 16, OPG-07, OPG-10, OPG-11, OPG-19, OPD19 and OPE-09 while the lowest polymorphism (38.46%) was observed with primer OPA-01. PCR amplification of 14 ISSR primers generated a total of 4076 scorable bands with an average of 291 bands per primer. The PIC values ranged from 0.77 (UBC-845) to 0.94 (ISSR-10). The highest PIC value of ISSR-10 indicated its usefulness for genetic diversity analysis in ginger. The Jaccard’s similarity values for RAPD, ISSR and pooled data of RAPD+ISSR markers ranged from 0.60 to 0.92, 0.39 to 0.95 and 0.55 to 0.92, respectively. The dendrogram constructed using RAPD and ISSR markers distinguished all the ginger genotypes geographically, in which genotypes collected from Gujarat showed high level of variations as compared to IISR, Calicut accessions.
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How to cite this article:
Virali Antala, Subhash Narayanan, Nidhi Radadiya, Hemangini Chaudhari, Hiral Desai, Ghanshyam Patil. Assessment of genetic diversity by using RAPD and ISSR markers in ginger (Zingiber officinale Rosc.) genotypes. Int J Chem Stud 2019;7(4):363-372.
