Vol. 7, Issue 4 (2019)
Molecular identification and in silico characterization of coat protein in chilli leaf curl virus associated in chilli from south Gujarat region of India
Author(s): Prachi Mistry, RK Kalaria and AI Patel
Abstract: Chilli leaf curl disease (CLCVD) incited in different species of crops and one of the most significant biotic disease in limiting production and productivity in India and worldwide. In this study, Natural incidence of CLCVD disease on chilli was observed in March, 2019 at Gujarat, India. The infected plants revealed systemic viral symptoms as leaf curling and thickening and swelling of veins, stem twisted and stunted. Molecular methods were followed to detection and characterized coat protein (CP) gene of Chilli leaf curl virus using specified design primers. The purified PCR product (~900 bp) was sequenced and further analysed showed that the virus had 99.00% sequence identities and closest phylogenetic relationships with isolates of Chilli leaf curl virus-India isolate Ahmadabad segment DNA-A, complete sequence (JN663846.1),Therefore name as Chilli leaf curl virus isolate NAU Gujarat coat protein (AV1) gene and submitted to NCBI (MK955890.1). ChiLCV coat-protein sequence was computationally analysed for presence of conserved domain as Gemini super family and further modelled to predict structure using swiss model that further validated using Ramachandran plot showing 89.10% with 172 amino acid in most favourable region. Thus, the present study will be helpful to understand evolutionary classification and the molecular host pathogen interaction to develop robust resistant strategies against Gemini viruses, particularly ChiLCV in the nearby future.
Pages: 711-718 | 360 Views 85 Downloads
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How to cite this article:
Prachi Mistry, RK Kalaria, AI Patel. Molecular identification and in silico characterization of coat protein in chilli leaf curl virus associated in chilli from south Gujarat region of India. Int J Chem Stud 2019;7(4):711-718.
