Vol. 7, Issue 4 (2019)
Development of cell culture systems from the striped catfish Pangasianodon hypophthalmus (Sauvage, 1878)
Author(s): Sathiyanarayanan A, Vijay Sundar Deva G, Nirmal T, Arul Murugan M, Safna P and Kesavan S
Abstract: In this study we report the development of primary culture systems from the caudal fin, and heart, tissues of Pangasianodon hypophthalmus by explant outgrowth technique. The target tissue samples were aseptically excised from the healthy fingerlings of P. hypophthalmus and explanted and maintained in Leibovitz -15 medium supplemented with 15% Fetal Bovine Serum at an optimum temperature of 28 â°C. Heterogenous population of cells comprising fibroblastic and epithelial like morphology were observed from all the three explants. Confluent monolayer of fin and heart cells were observed at different time period post seeding and its attachment to the flask. The established primary cultures from the explants were trypsini zed using trypsin in the subsequent passages.
Pages: 1367-1370 | 302 Views 68 Downloads
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How to cite this article:
Sathiyanarayanan A, Vijay Sundar Deva G, Nirmal T, Arul Murugan M, Safna P, Kesavan S. Development of cell culture systems from the striped catfish Pangasianodon hypophthalmus (Sauvage, 1878). Int J Chem Stud 2019;7(4):1367-1370.
