Vol. 7, Issue 4 (2019)
In vitro mutagenesis in Carica papaya L. using oryzalin as a mutagen
Author(s): Suvalaxmi Palei, DK Dash and GR Rout
Abstract: Induction of variability using mutagens have been used for long by breeders A mutated cell in a mass of callus have a larger chance of surviving competition with other cells. The majority of the mutants produced will be solid if shoots are regenerated from a repeated subculture of mutated callus. Hence, mutation studies in papaya were conducted with callus and soot tips. Highest multiple shoots has been obtained from 3.0 mg/l BAP, 0.50 mg/l IAA, 1.0 mg/l NAA. Shoot tips treated with oryzalin showed reduced survival percentage with increase in concentration and duration of oryzalin treatment. It was least at 50 µM oryzalin (17.6%) and the highest (70.0%) with control. LD50 for oryzalin treatment of shoot tip was estimated to be 22.7 µM. Treated shoot tips on multiplication medium (MS + 3.0 mg/l BAP,0.50 mg/l IAA and 1.0 mg/l NAA) stimulated shoot initiation from the cut end of the shoot apices at lower doses which decreased with increase in oryzalin concentration.
Pages: 1464-1466 | 287 Views 61 Downloads
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How to cite this article:
Suvalaxmi Palei, DK Dash, GR Rout. In vitro mutagenesis in Carica papaya L. using oryzalin as a mutagen. Int J Chem Stud 2019;7(4):1464-1466.
