Vol. 7, Issue 4 (2019)
In vitro shoot regeneration and shoot tip necrosis in prunus necrotic ring spot virus free peach cv. July Elberta
Author(s): Shelly Kapoor and Anil Handa
Abstract: The studies were conducted to develop a protocol for regenerating prunus necrotic ring spot virus (PNRSV) free shoots in peach cv. July Elberta using axillary buds as explants but in vitro propagation technique encountered serious problems due to high incidence of shoot tip necrosis (STN) during regeneration of peach cv. July Elberta. Axillary buds from field grown plants were used as explants and cultured on MS supplemented with different concentrations of BA and GA3. The findings revealed that the synergistic effect of combination of BA (0.5 mg/l), GA3 0.1 mg/l with casein hydrolysate (100 mg/l) or malt extract (100 mg/l) proved to be better for shoot regeneration. Frequent subculturing of nodal explants from in vitro derived shoots increases the number of multiple shoots, but the regenerated shoots exhibited the symptoms of shoot tip necrosis (STN). The findings of the present studies have clearly indicated that various factors such as strength of the media, different carbon sources activated charcoal and different calcium sources need to be investigated for reducing the incidence of shoot tip necrosis during in vitro shoot regeneration in tissue culture of peach cv. July Elberta.
Pages: 2134-2138 | 231 Views 41 Downloads
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How to cite this article:
Shelly Kapoor, Anil Handa. In vitro shoot regeneration and shoot tip necrosis in prunus necrotic ring spot virus free peach cv. July Elberta. Int J Chem Stud 2019;7(4):2134-2138.
