Vol. 7, Issue 4 (2019)
Study of stripe rust (Puccinia striiformis Westend. f. sp. tritici) severity of Bread wheat and validation of resistance in identified lines with the help of molecular marker
Author(s): Shivani, Avinash, JP Jaiswal and Bhatt N
Abstract: Wheat (Triticum aestivum L.) is an annual monocot cereal belonging to the family Poaceae (earlier gramineae), originated from south west Asia. The field experiments were conducted at Norman E. Borlaug Crop Research Centre of Govind Ballabh Pant University of Agriculture and Technology, Pantnagar during 2015-2016 and 2016-2017 in Rabi season. The experimental material consists of ninety elite lines from Indo-Australian project on triple rust resistance. The lines were evaluated in field conditions for stripe rust. These lines were categorized into resistance and susceptible on the basis of modified Cobb’s scale. Disease severity was noted at different intervals and Area under disease progress curve (AUDPC) was generated to calculate A-value. The laboratory work was done in order to confirm the presence of effective rust resistant gene(s) with linked molecular markers in elite lines. The SSR markers, Psp 3000 linked to effective yellow rust resistance gene Yr10.In the field experiment the lines, namely, P-2, P-3, P-46, P-62, P-73 and P-90 for stripe rust showed low coefficient of disease level (CDL), low A-value and low rate of infection with moderately resistance and moderately susceptible type of response. The use of molecular markers confirmed the presence of Yr10 gene in 14 lines. The lines possessing resistant genes and showing phenotypic resistance in field conditions can be used in breeding programme for developing resistance wheat varieties.
Pages: 2213-2221 | 298 Views 61 Downloads
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How to cite this article:
Shivani, Avinash, JP Jaiswal, Bhatt N. Study of stripe rust (Puccinia striiformis Westend. f. sp. tritici) severity of Bread wheat and validation of resistance in identified lines with the help of molecular marker. Int J Chem Stud 2019;7(4):2213-2221.
