Vol. 7, Issue 4 (2019)
Endophytic bacteria colonizing maize (Zea mays L.) exhibiting plant growth promoting traits and influence on plant growth
Author(s): Premsing Shivsing Marag and Archna Suman
Abstract: Endophytes are microbial symbionts residing within the plant for the majority of their life cycle without any detrimental impact to the host plant. Some endophytes are able to promote the growth of plants by various PGP mechanisms. This study envisages the isolation of endophytic bacteria, morphological characterization, in vitro screening for their plant growth promoting traits and in planta assay for their influence on plant growth. A total of 106 morphologically distinguishable bacterial endophytes were isolated from composite maize variety, Pusa Composite-4 (PC-4) from surface sterilized root, stem and leaf tissues on different growth media at vegetative, flowering and maturity stages of growth. The maximum population of endophytic bacteria was found at flowering stage in root. 62 isolates out of 106 isolates exhibited one or more plant growth promoting traits. P, K, Zn solubilization activity was found in 19, 3 and 30 isolates respectively; phytohormone IAA production tested positive in 15 isolates, whereas 27 isolates were siderophore producer. Five isolates produced HCN and 3 isolates were biological nitrogen fixer. Biocontrol activity was tested positive in 6 isolates against Exerohilum turcicum and one isolate against Rhizoctonia solani. Primarily 54.83% isolates were having single PGP trait, 29.03% double, 9.67% triple, 4.83% four and 1.61% six PGP traits. Selected PGP isolates showed positive effects on germination %, shoot dry weight and root dry weight ranging from 91 to 96%, 1.9 to 4.2 gm plant−1 and 0.5 to 1.98 gm plant−1respectively, as compared to the un-inoculated control. Application of such PGP endophytes appears to be one of the best options in enhancing biomass yield and improving crop productivity.
Pages: 2825-2832 | 257 Views 74 Downloads
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How to cite this article:
Premsing Shivsing Marag, Archna Suman. Endophytic bacteria colonizing maize (Zea mays L.) exhibiting plant growth promoting traits and influence on plant growth. Int J Chem Stud 2019;7(4):2825-2832.
