Vol. 7, Issue 4 (2019)
In vitro plant organogenesis in sweet orange cv. Mosambi by using epicotyl segments as explants
Author(s): Amardeep Kour and HS Rattan Pal
Abstract: By genetic transformation techniques, the exogenous genes can be introduced into the plants, but an effective tissue culture protocol with high regeneration percentage is important for genetic transformation. The present study was carried to strandaridzed a plant regeneration protocol for sweet orange (Citrus sinensis Osbeck cv. Mosambi) which can be used in plant transformation. Surface sterilization of seeds was carried out by using 0.1% HgCl2 at different time intervals and best results were obtained with 0.1% HgCl2 for 5 minutes exposure time. The seeds were allowed to germinate in vitro and were maintaining in the dark for three weeks, followed by one week at 16h photoperiod at 27 ± 2 oC. For shoot induction the epicotyls segments were cultured on MS medium with different concentrations of BAP and after adventitious bud growth, the shoots were transferred to full strength MS and half strength MS medium with different concentrations of NAA or IBA for rooting. The best results for morphogenetic responses were obtained with 1 mg l-1 BAP and for rooting the ½ MS medium supplemented with 1 mg l- IBA was the best. The protocols presented in this work will be suitable for with genetic transformation experiments for this cultivar.
Pages: 3106-3111 | 205 Views 60 Downloads
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How to cite this article:
Amardeep Kour, HS Rattan Pal. In vitro plant organogenesis in sweet orange cv. Mosambi by using epicotyl segments as explants. Int J Chem Stud 2019;7(4):3106-3111.
