Vol. 7, Issue 5 (2019)
Enhanced chitinase production ability and morphological variation of Trichoderma asperellum Mutants
Author(s): Rahul S Shete, Dr. ST Ingle, HM Totawar and AG Gathe
Abstract: Genetic modification using mutagenesis leads to enhance the potential for producing improved bioprotection is likely to trigger biocontrol by Trichoderma asperellum. Cobalt- 60 gamma radiation were used to induce mutation in Trichoderma asperellum. Chemical Mutagenesis was carried out by application of different concentrations of Ethyl methane sulfonate (EMS). In order to check the stability, mutants were grown up to seven generations on PDA and Variation in the morphological and cultural character were found in mutants as compared to mother culture. These mutants were tested for their chitinase productivity. TaMG 3 (T3), TaMG 4 (T4), TaME 4 (T15) and TaMG 7 (T7) reported to be the highest producer of chitinase enzyme, since it produced 0.70, 0.67, 0.66 and 0.66 chitinase enzyme units/ mg of protein respectively. Mother culture TaMC contained only 0.39 66 chitinase enzyme units/ mg of protein.
Pages: 183-188 | 494 Views 74 Downloads
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How to cite this article:
Rahul S Shete, Dr. ST Ingle, HM Totawar, AG Gathe. Enhanced chitinase production ability and morphological variation of Trichoderma asperellum Mutants. Int J Chem Stud 2019;7(5):183-188.
