Vol. 7, Issue 5 (2019)
Characterization of biosynthesised copper nanoparticle from Citrus sinesis and in-vitro evaluation against fungal pathogen Colletotrichum capsici
Author(s): Pandurang R Divte, Sarika Shende, Omkar M Limbalkar and Rohan A Kale
Abstract: Nanotechnology, a new emerging and fascinating field of science which permits advanced research in many areas with novel applications in the field of biotechnology and agriculture. In present studies, copper nanoparticles (CuNPs) biosynthesized from orange (citrus sinesis) juice act as a reducing agent, mixed with copper sulphate solution and boiled at 60-80 °C upto deposition of nanoparticles on inner wall of aluminium vessel. Later, the synthesized CuNPs were characterized by using UV-Vis spectrophotometer, Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), Particle size analyzer and Malvern zeta sizer. The size of CuNPs were 1-3 nm with an average size of 2nm analyzed using TEM, Particle size analyzer measured the total concentration of CuNPs were 20.91 particles/frame and 0.81E8 particles/ml. The zeta potential value of biosynthesized CuNPs was -4.55 mV. CuNPs solution of different concentration like 30, 50, 70, 100, 150, 200 ppm used for testing antifungal activity against Colletotrichum capsici. We found that, Colletotrichum capsici was more susceptible to the action of copper nanoparticle at higher concentration than lower concentration. The maximum activity of CuNPs was found against Colletotrichum capsici at concentration 200 ppm (28.00±081mm diameter), while the minimum activity was found at concentration 30 ppm (9.75± 2.06 mm diameter). The results of the disc diffusion method revealed that copper nanoparticles possess potential antifungal activity. In future, biosynthesized CuNPs could be useful for formulations of agriculturally important pesticides for the effective control of chilli anthracnose.
Pages: 325-330 | 354 Views 52 Downloads
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How to cite this article:
Pandurang R Divte, Sarika Shende, Omkar M Limbalkar, Rohan A Kale. Characterization of biosynthesised copper nanoparticle from Citrus sinesis and in-vitro evaluation against fungal pathogen Colletotrichum capsici. Int J Chem Stud 2019;7(5):325-330.
