Vol. 7, Issue 5 (2019)
ABA dependent candidate gene identification and diversity analysis through SSR in sugarcane (Saccharum officinarum L.) under salinity stress
Author(s): Ranjana Singh and RS Sengar
Abstract: Eleven (11) abscisic acid (ABA) dependent gene specific primer and twenty five (25) simple sequence repeats (SSR) primer pairs were chosen to detect the presence of gene responding under salinity stress and polymorphism to the 10 sugarcane accessions respectively. Out of 11 only 6 ABA denendent gene primer got amplified. The highest PIC (polymorphism information content) 0.764 for the primer RGS1 and lowest PIC for primer CDPK 0.22 was recorded. The resolving power were obtained in the range of 0.80 to 1.60. In case of SSR out of 25 primer only 19 were got amplified and generated 67 reproducible bands, to which 52 were polymorphic and 13 were monomorphic. The average number of polymorphic bands was 2.73 per primer, polymorphism range from 66.67% to 100%. The highest PIC 0.99 was recorded for the primer UGSM507 while lowest PIC 0.16 was recorded for the primer UGSM695, with resolving power ranging from 0.20 to 1.80. Similarity value for all the 10 genotypes ranged from 0.58 to 1.00. The minimum similarity exhibited by genotype Co 0238, Co 8279 and Co 0118, whereas the maximum similarity was shown by the genotype Co 5011 and CoS 7250.
Pages: 1291-1297 | 268 Views 56 Downloads
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How to cite this article:
Ranjana Singh, RS Sengar. ABA dependent candidate gene identification and diversity analysis through SSR in sugarcane (Saccharum officinarum L.) under salinity stress. Int J Chem Stud 2019;7(5):1291-1297.
