Vol. 7, Issue 5 (2019)
Studies on entomopathogenic fungus Metarhizium anisopliae isolated from Spodoptera litura
Author(s): BD Sonune, MJ Patil, RB Kothikar, HR Sawai, VS Wargane and Kajal Mane
Abstract: The laboratory experiment was carried out at laboratory of Plant Pathology section, College of Agriculture, Nagpur during 2018-19. Isolates of Metarhizium anisopliae were collected, studied and evaluated for its morphological and cultural studies on four different media viz., PDA, SDA, SDAY and SMA. The cultural studies revealed that, SDAY media support maximum linear growth (35.00 mm) and abundant sporulation to M. anisopliae followed by SMA, SDA and PDA exhibiting 30.83, 29.50 and 25.33 mm growth, respectively. Hence, SDAY media was selected as basal medium for further studies. It was noticed that, the requirement of temperature for maximum growth and sporulation of M. anisopliae fungus was observed at 25 0C (35.00 mm) and 30 0C (34.50 mm) followed by room temperature (30.50 mm). The fungus could tolerate15-35 0C. However, it could not grow at 400C. All the different humidity levels supported growth and sporulation of M. anisopliae. The maximum linear growth was recorded at 95 per cent RH (35.50 mm) with abundant sporulation. Among the different pH levels, pH 5.5 was found to be the best level for growth and sporulation (35.33 mm). It indicates that, the maximum biomass at low pH and least biomass growth at higher basic pH.
Pages: 1681-1685 | 244 Views 61 Downloads
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How to cite this article:
BD Sonune, MJ Patil, RB Kothikar, HR Sawai, VS Wargane, Kajal Mane. Studies on entomopathogenic fungus Metarhizium anisopliae isolated from Spodoptera litura. Int J Chem Stud 2019;7(5):1681-1685.
