Vol. 7, Issue 5 (2019)
Molecular characterization of Fusarium oxysporum f. sp. melongenae (Schlecht) mutuo and Ishigami in Marathwada region of Maharashtra by using ITS-RFLP Markers
Author(s): V Govardhan Rao, DN Dhutraj, SR Bhalerao, KT Apet, CV Ambadkar, B Prasanna Kumar, AT Daunde, PL Sontakke and AG Patil
Abstract: ITS-RFLP (Internal Transcribed Spacer-Restriction Fragment Length Polymorphism) marker were used to study the genetic variability among Fusarium oxysporum f. sp. Melongenae isolates from different regions of Marathwada collected during the summer 2018-2019. A total 2 restriction enzymes were used for the digestion of ITS region of the Fom isolates Study of ITS-RFLP analysis for genetic diversity shows the genetic relationship between the 8 Fom isolates. The major cluster I consisted of seven isolates viz. Fom-1 (Soegaon), Fom-2 (Ambajogai), Fom-4 (Badnapur), Fom-5 (Chakur), Fom-6 (Kinwat), Fom-7 (Tuljapur) and Fom-8 (Gangakhed) while second major cluster II comprises single isolates Fom-3 (Sengaon) shown 33% genetic similarity with cluster I and genetic diversity 67% with all isolates.
Pages: 2319-2326 | 235 Views 70 Downloads
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How to cite this article:
V Govardhan Rao, DN Dhutraj, SR Bhalerao, KT Apet, CV Ambadkar, B Prasanna Kumar, AT Daunde, PL Sontakke, AG Patil. Molecular characterization of Fusarium oxysporum f. sp. melongenae (Schlecht) mutuo and Ishigami in Marathwada region of Maharashtra by using ITS-RFLP Markers. Int J Chem Stud 2019;7(5):2319-2326.
