Vol. 7, Issue 5 (2019)
Morphological variation among different isolates of Colletotrichum gloeosporioides isolated from major fruit crops in Khandesh region of Maharashtra
Author(s): KS Chaudhari, SV Kolase, RV Patil, MA Barate, MP Khatal and SM Jare
Abstract: Present investigation reveals the study of morphological variation among different isolates of Colletotrichum gloeosporioides isolated from various crops. Twelve diseased specimens were collected from different district of Khandesh region of Maharashtra and subjected to tissue isolation on PDA. Out of 12 specimens obtained from different hosts, 12 isolates from 3 isolate from pomegranate, 3 isolate from sweet orange, 3 isolate from banana and 3 isolates from guava from three different district of Khandesh region of Maharashtra. These 12 isolates from 4 major fruit crops were found to be pathogenic when inoculated on respective plant hosts. These 12 isolates were used for further study and further abbreviated as CgPD1, CgPJ 2, CgPN 3, CgSD 4, CgSJ 5, CgSN 6, CgBD 7, CgBJ 8, CgBN 9, CgGD 10, CgGJ 11 and CgGN 12. Colletotrichum gloeosporioides isolated differed significantly in all morphological traits except the type of mycelium. Mycelium of all isolates was septate. Maximum average mycelial growth rate of 12.85 mm / day was observed in isolates CgPD 1 which isolated from pomegranate fruit crops. The mycelial width of all 12 isolates ranged between 4.11 – 3.1 µ. In general, conidia were hyaline, cylindrical to allantoidal with round ends. The highest average conidial length was exhibited by isolate CgPJ 2 (11.75 µ) infecting pomegranate and it was at par with CgSD 4 (11.70 µ) while, shortest length of conidia were observed in CgGN 12 (6.88 µ) infecting guava and it was at par with CgGJ 11 (6.96 µ) and CgGD 10 (7.42 µ). There was significant variation in L X B ratio conidia of CgBD 7 were thin and narrow as the L X B ratio was maximum (4.70 µ) were from banana, while it was minimum (2.57 µ) in CgSD 4 these isolates were from sweet orange. Among the 12 isolates under study, 9 isolates were produced acervuli in the culture. Isolates CgPN 3, CgSJ 5 and CgGJ 11 never produced acervuli in culture. The average maximum length of the setae was (75.82 µ) recorded in isolate CgBJ 8 and it was followed by CgBD 7. The shortest setae were observed in CgBN 9 having average length of (27.03 µ) and it was on par with CgPJ 2, CgGN 12, CgSD 4 and CgGD 10. Out of 12 isolates, 9 isolates could produce setae as an integral part of acervulus. Acervuli of isolates CgPN 3, CgSJ 5 and CgGJ 11 were without setae. The average maximum length of the setae was (75.82 µ) recorded in isolate CgBJ 8 and it was followed by CgBD 7. The shortest setae were observed in CgBN 9 having average length of 27.03 µ.
Pages: 2777-2782 | 201 Views 41 Downloads
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How to cite this article:
KS Chaudhari, SV Kolase, RV Patil, MA Barate, MP Khatal, SM Jare. Morphological variation among different isolates of Colletotrichum gloeosporioides isolated from major fruit crops in Khandesh region of Maharashtra. Int J Chem Stud 2019;7(5):2777-2782.
