Vol. 7, Issue 5 (2019)
Characterization of chickpea (Cicer arietinum L.) genotypes through morphological and RAPD analysis
Author(s): Savita Budania, Manju K Choudhary, Payal Choudhary and Arunabh Joshi
Abstract: Fifteen Chickpea (Cicer arietinum L.) genotypes were evaluated for genetic diversity using morphological traits and RAPD markers. Eight morphological traits were studied and subjected to analysis of variance. Mean squares due to genotypes were highly significant as well as wide mean range performance was observed for germination percentage, plant height, effective pods per plant, test weight and seed yield. Ward’s cluster analysis based on morphological traits separated the accessions into two groups. RAPD analysis was carried out with 16 primers. They were screened but only 11 primers produced amplification. These 11 primers produced 76 bands, in which 64 were polymorphic bands. Average polymorphism was 84.2 percent. Based on the RAPD markers, a dendrogram was constructed using the UPGMA method. The similarity coefficient ranged from 0.51 to 0.88 with an average of 0.70. Cluster analysis based on RAPD data separated the accession into five main groups. The trends of genotypes relationship amongst the chickpea determined by RAPDs were consistent with their morphological traits.
Pages: 2783-2789 | 296 Views 59 Downloads
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How to cite this article:
Savita Budania, Manju K Choudhary, Payal Choudhary, Arunabh Joshi. Characterization of chickpea (Cicer arietinum L.) genotypes through morphological and RAPD analysis. Int J Chem Stud 2019;7(5):2783-2789.
