Vol. 7, Issue 5 (2019)
Studies on rooting and hardening of in vitro plantlets of carnation (Dianthus caryophyllus L.)
Author(s): Bharti Gautam, Puja Sharma and YC Gupta
Abstract: The present research work involves rooting and hardening in carnation (Dianthus caryophyllous). The experiments were laid out in a Completely Randomized Design (factorial) consisting of two cultivars i.e. ‘Parendillo’ and ‘Yellow Star’. The in vitro multiplied shoots were separated and individual shoots were transferred to MS medium supplemented with different levels of auxins (NAA/IBA) with or without 0.1% activated charcoal for rooting of in vitro multiplied shoots. The concentration of both NAA and IBA varied from 1-2 mgl-1. Per cent rooting, days taken for visible root formation, length of the longest root (cm) and number of root/shoot were recorded 4 weeks after culturing. MS medium containing 2 mgl-1 NAA and 0.1% activated charcoal was found to be the best for in vitro rooting of multiplied shoots. The rooted plantlets were then planted in different hardening media in disposable cups and watered immediately with fine mist of spray. These cups were then covered with the misted pricked transparent plastic bag to ensure high humidity. Pricking of plastic bag was done to maintain aeration. The in vitro rooted plantlets showed 100% survival and maximum growth of plantlets hardened in a mixture of cocopeat : sand : perlite (2:1:1, v/v).
Pages: 3179-3182 | 218 Views 56 Downloads
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How to cite this article:
Bharti Gautam, Puja Sharma, YC Gupta. Studies on rooting and hardening of in vitro plantlets of carnation (Dianthus caryophyllus L.). Int J Chem Stud 2019;7(5):3179-3182.
