Vol. 7, Issue 5 (2019)
In vitro cell selection and its molecular confirmation in tomato against buckeye rot (Phytophthora nicotianae var. parasitica)
Author(s): Abhishek Kumar, Shilpa, Rajinder Kaur and Monica Sharma
Abstract: Among various fungal diseases buckeye rot is a major constraint in tomato cultivation and the disease is present in almost all the tomato growing areas of the world. The aim of present study was to evaluate the effectiveness of in vitro test in which culture filtrate of Phytophthora nicotianae var. parasitica was used to select the tolerant calli against buckeye rot disease. Murashige and Skoog medium supplemented with 3.0 mg/l Naphthalene acetic acid (NAA) + 1.0 mg/l 6-Benzylaminopurine (BAP) was used for callus induction and multiplication. The calli derived from the cotyledon explant of in vitro raised aseptically grown seedling were selected against the different concentrations (1-25%) of culture filtrate of Phytophthora nicotianae var. parasitica. Calli survival at 20% as selective dose of culture filtrate was 30.64% and survived calli were subcultured on multiplication medium (NAA 3mg/l + BAP 1mg/l) without culture filtrate. After multiplication, the calli were again culture on 20% selective medium and this process was repeated two to three times and tolerant calli were selected. The selected calli were transferred to the previously standardized regeneration medium (MS medium supplemented with 2.75, 3.0 mg/l BAP). Initially the callus turned to slightly raised green nodulation on this growth regulator which later leads to shoots formation. Molecular studies revealed that the selected tolerant calli were different at genomic level from the normal callus and it ascertains that indeed the resistance adaptation observed is due to genetic changes. Finally, it was concluded that resistant plantlets regenerated from tomato cv. Solan Vajr callus can be used for cell selection and will benefit the farmers to encounter with huge crop losses due to buckeye rot.
Pages: 3278-3283 | 188 Views 55 Downloads
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How to cite this article:
Abhishek Kumar, Shilpa, Rajinder Kaur, Monica Sharma. In vitro cell selection and its molecular confirmation in tomato against buckeye rot (Phytophthora nicotianae var. parasitica). Int J Chem Stud 2019;7(5):3278-3283.
