Vol. 7, Issue 6 (2019)
Method validation of residual cyanide determination in lamotrigine API using sensitive spectrophotometric method
Author(s): Divya Uthaman, Smita, Pranshu Kapoor, Rakhi Singh, ML Aggarwal and KM Chacko
Abstract: Lamotrigine is known as an anticonvulsant or antiepilectic drug. Trace level of cyanide may remain in the product in cyanation step during manufacturing process. Based on the principle of sensitive colorimetric procedure, converting the product to cyanogen chloride, CNCl by reaction with chloramine-T and subsequently the formation of colored complex Quinquevalent Cyanide with pyridine-barbituric acid reagent, the residual cyanide content was determined. The method was validated for various parameters such as System suitability, Linearity, Method Precision, Accuracy and Intermediate precision by employing UV-Spectrophotometer. A wide linear range concentration of 0.015-0.1 mg/Kg was observed with r2 values ≥ 0.99. The sample was spiked at concentration level of 0.025; 0.05; 0.075 and 0.1 mg/Kg respectively and better recoveries between 80-120% with the acceptable relative standard deviation (RSD) i.e. <20% were obtained. The proposed technique efficiently screens residual cyanide in Lamotrigine API as residual Cyanide.
Pages: 1069-1072 | 501 Views 129 Downloads
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How to cite this article:
Divya Uthaman, Smita, Pranshu Kapoor, Rakhi Singh, ML Aggarwal, KM Chacko. Method validation of residual cyanide determination in lamotrigine API using sensitive spectrophotometric method. Int J Chem Stud 2019;7(6):1069-1072.
