Vol. 7, Issue 6 (2019)
Study of genetic diversity in mungbean (Vigna radiata L. Wilczek) cultivars using RAPD markers
Author(s): Avinash Varma, SP Mishra and Ajay Tripathi
Abstract: A set of twelve cultivars of mungbean [Vigna radiata (L.) Wilczek] were taken as experimental materials in the present study. The young leaves were collected from five individual plants and leaf samples were pooled for each accession. Random amplified polymorphic DNA (RAPD) markers were used to study the DNA polymorphism in twelve Indian mungbean cultivars. A total of 17 random primers were used in the research and 11 of them generated reproducible RAPD patterns. Out of 17 random primers, a total of 11 highly polymorphic primers were used for amplification of genomic DNA isolated from 12 mungbean cultivars in this study. Thus the result is based on the amplification products of 11 polymorphic primers with 12 cultivars of mungbean. Amplification of genomic DNA of 12 Indian mungbean cultivars with 11 RAPD primers yielded 152 fragments that could be scored, of which 108 were polymorphic, thus with average number of bands amplified per primer was 13.8 and with an average of 9.8 polymorphic fragments per primer. Number of amplified fragments with random primers ranged from 8 (OPA 14) to 22 (OPA11). Percentage polymorphism ranged from 23.08% (OPA09) to a maximum of 100% (OPN 05 and OPN 016), with an average of 71.05%. The Jaccard’s similarity coefficients matrix based on RAPD profiles were subjected to UPGMA cluster analysis. The RAPD cluster pattern segregated the 12 mung bean cultivars into two major clusters. The cultivar Pusa-0672 formed Cluster 1 (C-1) and cultivars Samrat, BPMR-145, HUM-2, MUM-2, Pairy Mung, Pant M-2, Pant M-4, PKV AKM-4, Pusa-9072, RMG-62 and RMG-268 have made Cluster 2. This indicated the narrow genetic base in the Indian mung bean cultivars used in the study.
Pages: 1139-1145 | 291 Views 61 Downloads
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How to cite this article:
Avinash Varma, SP Mishra, Ajay Tripathi. Study of genetic diversity in mungbean (Vigna radiata L. Wilczek) cultivars using RAPD markers. Int J Chem Stud 2019;7(6):1139-1145.
