Vol. 7, Issue 6 (2019)
Prospecting inexpensive media for mass production of microbial inoculants at farmers field
Author(s): Niharika Bharti, Geeta Kumari, Devendra Singh, Manindra Nath Jha, Navnit Kumar and Sunita Kumari
Abstract: Utilization of beneficial and multifaceted microorganism can solve the problem of getting enhanced yield without disturbing the ecosystem. Timely availability of quality microbial inoculants is one of the major concerns of the farming community. Thus, we aimed to design a media based on household wastes for mass culturing of beneficial microbes especially symbiotic nitrogen fixer Mesorhizobium spp. and multifaceted Bacillus spp. This work was initiated with the selection of suitable substrate from home waste along with carbon supplements source that can be available at village level. Standard media like Yeast extract mannitol for Mesorhizobium and Nutrient yeast salt medium for Bacillus was included as a reference media. Components of newly designed media include boiled rice water, pulse/ vegetables wash and different sources of carbon viz. table sugar, jaggery and glucose. Boiled rice water without any carbon supplementation did not support the growth of targeted microbes. Different concentration of boiled rice water along with dal/vegetables wash and sugar supported the growth of targeted microbes based on visual observation. Among carbon sources, glucose was observed to support maximum growth having optical density of 1.621 and cfu was 170x106. However, The best combination for getting maximum targeted biomass was 58% rice boil water, 10% pulse wash, 10% vegetable wash, 20% water, 2% glucose. The proposed media is highly cost effective and providing high yield of microbial biomass and its combination are available at village level. Designing of media will facilitate the farming community for the production of the microbial inoculants at their site as and when required.
Pages: 1435-1442 | 303 Views 66 Downloads
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How to cite this article:
Niharika Bharti, Geeta Kumari, Devendra Singh, Manindra Nath Jha, Navnit Kumar, Sunita Kumari. Prospecting inexpensive media for mass production of microbial inoculants at farmers field. Int J Chem Stud 2019;7(6):1435-1442.
