Vol. 7, Issue 6 (2019)
Comparative analysis of quantitative gene expression of prolactin, leptin and glutathione peroxidase in buffalo calves supplemented with different yeast derivatives
Author(s): Virendra Kumar Singh, Sandhya S Chaudhary and Tanvi D Manat
Abstract: The present study was conducted on 9 apparently healthy Surti young buffalo female calves aged (6-12 months) divided into 3 groups of 3 each categorized as group I (control), group II (supplemented with rumen specific yeast Saccharomyces cerevisiae CNCM I-1077 @ 4x109 cfu/animal/day and group III (supplemented with selenoyeast-inactivated yeast Saccharomyces cerevisiae containing selenomethionine ensuring dietary inclusion of selenium @ 0.3 ppm). Whole blood was collected at day 0 of study (beginning), day 45 and day 90 of study (end). Total RNA was isolated, cDNA was synthesized and quantitative gene expression of prolactin, leptin, and GPx1 was studied using real time PCR. Downregulation of prolactin was observed in all groups at all stages wherein between groups, down regulation during 0-45 days was maximum in group II and minimum in group III.; during 45-90 days maximum in group III and minimum in group II and during 0-90 days between groups the down regulation was maximum in group II and III followed by control.Within groups down regulation increased in group I and III and decreased in II from 0-45 days to 45-90 days of study. Between the groups leptin was upregulated during 45-90 days and 0-90 days of study. Maximum upregulation was in group II and minimum in control. During 0-45 days the upregulation was almost similar in all the groups. Within groups the upregulation increased in group II and decreased in group III and group I from 0-45 days to 45-90 days of study. GPx1 was upregulated in all groups and at all stages but between groups was maximum in group III. Thus it was concluded that in female Surti buffalo calves supplementation of rumen specific yeast had more positive influence on prolactin and leptin gene expression whereas selenoyeast supplementation positively impacted glutathione peroxidase expression.
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How to cite this article:
Virendra Kumar Singh, Sandhya S Chaudhary, Tanvi D Manat. Comparative analysis of quantitative gene expression of prolactin, leptin and glutathione peroxidase in buffalo calves supplemented with different yeast derivatives. Int J Chem Stud 2019;7(6):1898-1901.
