Vol. 7, Issue 6 (2019)
Nutrigenomic effects of different yeast derivatives supplemented in surti buffaloes during early lactation
Author(s): Virendra Kumar Singh, Sandhya S Chaudhary and Tanvi D Manat
Abstract: The present study was conducted on nine postpartum Surti buffalo divided into 3 groups of 3 each categorized as group I (control), group II (supplemented with rumen specific yeast Saccharomyces cerevisiae CNCM I-1077 @ 10x109 cfu/animal/day and group III (supplemented with selenoyeast-inactivated yeast Saccharomyces cerevisiae containing selenomethionine ensuring dietary inclusion of selenium @ 0.3 ppm). Whole blood was collected at day 0 of study (beginning), day 45 and day 90 of study (end). Total RNA was isolated, cDNA was synthesized and quantitative gene expression of prolactin, leptin, and GPx1 was studied using real time PCR.
Due to rumen specific yeast supplementation the relative expression of prolactin gene between groups in group II was upregulated more than of control. In group III the downregulation for prolactin between groups was lesser than group I during both 0-45, 45-90 and 0-90 days. Leptin expression was more upregulated in group II (yeast supplemented) than group I (control).The leptin expression in group III between groups was slightly more downregulated during 0-45 and 0-90 day and slightly less upregulated during 45-90 day than in group I. The expression of GPx1 revealed that upregulation was present in all groups and stages postpartum in Surti buffaloes. Between groups it was higher in group II than I. The upregulation of GPx1 between groups was more in group III than in group I during all stages.Thus it was concluded that in Surti buffalo during early lactation supplementation of rumen specific yeast had favourable nutrigenomic effects on prolactin and leptin gene expression whereas selenoyeast supplementation impacted positively the expression of glutathione peroxidase gene.
Pages: 1926-1930 | 265 Views 67 Downloads
download (7218KB)
How to cite this article:
Virendra Kumar Singh, Sandhya S Chaudhary, Tanvi D Manat. Nutrigenomic effects of different yeast derivatives supplemented in surti buffaloes during early lactation. Int J Chem Stud 2019;7(6):1926-1930.
